| DOI LINK: https://doi.org/10.59671/gvis9 |
| Paper ID:gvis9 |
| Volume:404 |
| Issue:5 |
| Title:Optimizing Equilibration Protocols through Ubiquinone Supplementation in Goat Frozen Semen Diluent |
| Abstract:Equilibration period is urgently required for sperm�s adjustment to the diluent preventing severe damage during cryopreservation. The elevation of lipid peroxidation during equilibration, indicating a potential involvement of oxidative stress, emphasizes the significance of optimizing equilibration protocols to minimize the detrimental effects of free radical production on sperm quality. The aim of this study is to identify the supplementation effect of adding ubiquinone into semen diluent on membrane integrity, motility, and survivability of goat spermatozoa following an hour of equilibration. The ubiquinone supplementation was combined under the circumstances as follows: The groups with the following supplementation doses: Group 2 (B) received a dose of 5 mg/dL; Group 3 (C) received a dose of 10 mg/dL; Group 4 (D) received a dose of 15 mg/dL; Group 5 (E) received a dose of 20 mg/dL; and Group 6 (F) received a dose of 25 mg/dL. Group A was the negative control group. Following a one-way ANOVA examination of the research data, additional Duncan tests were performed. The results of the study showed that the addition of ubiquinone had a significant (p<0,05) effect on the percentage of motility, viability, and membrane integrity of Kacang goat spermatozoa after an hour equilibration. According to the statistical analysis, the ideal concentration of ubiquinone in egg yolk skim milk for sustaining motility is 12,5 mg/dL. The best number for preserving the viability and membrane integrity of Kacang goat spermatozoa following equilibration, on the other hand, is 25 mg/dL. In conclusion, when ubiquinone is added to the frozen semen diluent, the quality of Kacang goat spermatozoa remains stable after equilibration. |
| Keywords:spermatozoa, equilibration, semen diluter, motility, viability, membrane integrity |
| Authors:Tatik Hernawati1*, Suherni Susilowati2, Sri Mulyati3, Yudit Oktanella4 |
| Paper PDF Link: View full PDF |