Abstract:Background and study aim: 14-3-3? protein involved into multiple cellular processes. The aim of this study was to investigate the role of 14-3-3? protein in colorectal cancer (CRC) and its relationship with tumor angiogenesis. We also used CRC cell lines HCT116 and DLD1 to assess the biological function of 14-3-3?.Material and methods: In this study, 72 colorectal cancer specimens were investigated by immunohistochemical staining for CD105 and 14-3-3?. 14-3-3? was enforced expressed in CRC cell lines, and the effect of 14-3-3? on the expression of p-mTOR and p-GSK-3ï¿½ was assessed by western blot.Results: The overexpression of 14-3-3? was found in 60 (83.3 %) colorectal cancer tissue samples. 14-3-3? expression in CRC was significantly associated with tumor differentiation (p= 0.001), pT stage (p= 0.001), and advanced pTNM stage (p= 0.002). CD105 overexpression were detected in 51 (70.8 %) CRC specimens. CD105 expression in CRC was significantly associated with tumor differentiation (p= 0.039), pT stage (p= 0.001), advanced pTNM stage (p= 0.000) and nodal status (p= 0.002). In addition, the overexpression of 14-3-3? was positively correlated with the expression of p-GSK-3ï¿½ and p-mTOR in DLD1 and HCT116 cells.Conclusions: Our study suggested that 14-3-3? overexpression accompanied with angiogenesis, and associated with colorectal carcinoma progression, and the mechanism was probable associated with p-GSK-3ï¿½ and p-mTOR signal pathway. Thus, targeting 14-3-3? and angiogenesis might be a new therapeutic strategy in colorectal carcinoma.
Abstract:In recent years, the trend for collaborative consumption has been developing in Indonesia. Collaborative consumption perceived as creating opportunities for entrepreneurs along with consumers by sharing goods, services, and information between individuals. The purpose of this study is to investigate factors that influence collaborative consumption by investigating perceived benefit, perceived risk, and technological factor as the antecedents to intention to participate in collaborative consumption. Questionnaires were distributed to 168 respondents consist of online transportation users of GoJek and Grab in the cities of Jakarta, Tangerang, Bekasi, and Depok. Data collected were analyzed using structural equation modelling through outer and inner method. The results indicated that all antecedents proven to have significant influence to the intention to participate in collaborative consumption, with technological factor being the greatest influence. The practical implications for Asian business from this study shows that collaborative companies, must take account user’s enjoyment through payment efficiency and security in the privacy of data.
Abstract:This study develops and validates a creative school environment test (CSET) for university students. After deducting the domain and components through a literature review, the content validity was confirmed by experts, and 20 question items in two domains were developed as a preliminary test. The participants of this study were 626 university students. The data were statistically processed using SPSS WIN 25.0 and AMOS WIN 25.0 programs to verify the reliability and validity of the test. The appropriateness of the Kaiser-Meyer-Olkin sample was confirmed, and Bartlett’s sphere formation test was performed. Exploratory factor analysis was performed using the main axis decomposition method and oblimin rotation, confirmatory factor analysis, the maximum likelihood method, and fitness indexes such as CFI, IFI, TLI, and RMSEA were used. The final decision regarding the creative school environment test for university school students was categorized into two domains, with a total of 18 questions, and the reliability of each area was Cronbach .871–.905, while the total reliability was .923. Therefore, the test developed in this study has the validity and reliability of measurement items for each area, and it can be used to measure the creative school environment for university school students.
Abstract:Prior studies focused on the relationships among the factors affecting the\nflipped classroom\'s learning performance (FCLP), but few analyzed the factors that\naffect FCLP. This study constructed a four-dimension model of the factors affecting\nFCLP based on the revised community of inquiry (RCOI) framework and tested it in\nthree flipped classrooms (N=231) in a leading Chinese university. The analysis revealed\nthat learners\' perception of FCLP was affected by the social presence, teaching presence,\ncognitive presence, and learning presence in descending influence order. The cognitive\npresence acted as a full mediator between learning presence and FCLP and a partial\nmediator among teaching presence, social presence, and FCLP. The conclusion may\nprovide a theoretical and practical guide to the future teaching design and class\norganization of flipped classrooms.
Abstract:Equity in territorial planning is is a wellknown concept and of central importance in the theoretical debate and planning practice of Western countries. However, in Albania, the goal of justice and equality in territorial planning remains a new challenge, unaddressed in practice and far from the focus of academic research. This paper seeks to raise concerns about the evaluation of new local plans that have been drafted over the last decade in Albania regarding the criteria of justice and equality for urban development. The term equality, in the context of city planning, refers to the technique of conforming the territory and the developing real estate, for which the regulatory plan should evenly distribute the advantages and disadvantages of urban planning.. We argue that there is a gap in the legal framework in Albania which does not provide a methodology that enables the fair distribution of development rights through territorial plans. The new local plans drafted from 2013 onwards use Development Instruments such as land use and territorial development indicators reflecting the factual situation in some areas and unsubstantiated interests in others. In this regard, there is a need for a review of local territorial plans regarding the evaluation of the implementation of the principle of equality in the distribution of development rights. In a further perspective, the article suggests an integrated review of the legal planning framework including the legal provisions of sectoral legislation governing informal construction issues and property titles unresolved by the political regime of past decades. Related to the issue of equality of development law with property development rights, the article argues that an integrated solution of the legal framework of territorial planning and development by applying the criteria of justice and equality in the distribution of urban development rights, offers opportunities for resolving conflicts inherited from other legal acts, widely contested in connection with the denial of the right to territorial development, such as the law on the treatment of property and the law on the legalization of informal settlements.
Abstract:Inulin is a polysaccharide with several applications within the chemical, pharmaceutical, and food industry. It is considered a dietary fibre that provides multiple health benefits. In this work, the yield of raw inulin obtained from garlic agro-industrial useless organic waste was maximized, by applying the response surface methodology in a central composite design (CCD), in which different distilled-water (DW)-to-garlic-agro-industrial-organic-waste (GAIOW) ratios (3 and 5 mL/g) and different temperatures (60 and 80 °C) were evaluated. Optimal condition was obtained with a DW/GAIOW ratio of 4.3 mL/g and a temperature of 80.2 °C. Under this condition, the quadratic model showed a maximum yield of crude inulin of 8.17 ± 0.89 g/100 g. Further, the CCD model obtained was validated with three additional experiments at the same optimal condition. The FTIR spectra of inulin obtained from garlic agro-industrial organic residues and chicory inulin showed similarities and differences, presumably related to the different degrees of polymerization of the fructans present.
Abstract:The educational process, assumed as the interaction between individuals of different generations, allows the transmission of relevant aspects for the intellectual formation and personal development of the human being. The purpose of this study was to determine the effect of a recreational game program on mathematics learning in third grade students; The research was carried out under the quantitative approach, with a quasi-experimental and longitudinal cut design where the `Instruimos` test was used as an instrument. The main results found were that both the control and the experimental group presented changes in the post-test, being significant in the experimental group. The aforementioned study concludes that the educational processes that use recreational play are positive since this allows the understanding of concepts, the improvement of techniques and the acquisition of problem solving methods.
Abstract:This study investigates the contribution of three categories of reading strategies to 15 year old school students’ mathematical literacy. The data for Singapore, Australia, the United States of America, and Saudi Arabia were obtained from the Programme for International Student Assessment (PISA 2018). The multiple regression results showed that all the three types of reading strategies contributed significantly to mathematical literacy with much variation between the sub-strategies. The summary writing strategy category contributed best to students’ mathematical literacy in Saudi Arabia, Singapore, and Australia with beta values, 17.7%, 13.7%, and 10%, respectively whereas the reaction to email category (focused on critical thinking) was the best contributed category in the USA (7.3%). The findings further illuminate which specific strategies most strongly predict mathematical literacy across all four countries, and which distinguish the country with the best mathematical literacy performance (Singapore) and the lowest performer (Saudi Arabia). The issue is further discussed whether reading strategies have a direct contribution on mathematical literacy or not and how the answer might affect possible intervention policies to improve mathematical literacy in schools.
Abstract:COVID-19 has emerged as multifarious lung disease with various clinical presentations in addition with common cold and flu. The airway inflammation, bronchial hyper responsiveness and mucus secretion leading to airflow obstruction is characterized as cause of inconvenient breathing. Currently the CoV-2 is diagnosed with polymerase chain reactions and some biochemical techniques employed in virology but lung function tests are ignored. No one is still established serum biomarker to assess the severity of inflammation and obstruction of airways. Present study was conducted to evaluate clinical utility of serum Periostin level in Cov-2 infected patients in term of significant correlation of serum Periostin levels with absolute Eosinophil count and lung function test. Total 760 CoV-2 infected participants were subjected to study. Infection was further classified into mild, intermittent, moderate and severe types and 100 patients for each groups were studied. Patients with COPD or other type of allergy were excluded from the study. Patients from 18-60 years of age, diagnosed with asthma by a Pulmonologist and age and gender matched non-asthmatic adults were included in the study bearing CoV-2 infections. Absolute eosinophil count were determined by XN-1000 Sysmex hematology analyzer and serum Periostin level were measured with manual Elisa method. Lung function test was performed with spirometer (FEV1) at Ayub Teaching hospital. The data was entered and analyzed using SPSS 20.0. Comparison between two groups were calculated by sample t-test and correlation were calculated by person correlation coefficient. Study concluded that Serum periostin level and absolute eosinophil count were significantly correlated with severity of COVID-19 infections and can be used as surrogate markers for diagnosis and severity of disease.